Systematic Development of a Detergent Toolbox as an Alternative to Triton X-100

In biomanufacturing, detergents are essential for inactivating enveloped viruses during protein purification. While Triton X-100 has long been the industry standard, its environmental toxicity to aquatic life has driven the need for safer solutions. Our team has successfully identified and rigorously evaluated two alternative detergents that match Triton X-100’s effectiveness while offering a more sustainable profile. This breakthrough provides us with practical, environmentally responsible options for viral inactivation in protein therapeutic production.