XS® Pichia and E.coli Expression Systems

The XS® Pichia & E.coli Expression Systems

The XS Technologies® microbial platform contains the microbial expression systems for today’s therapeutics. With good yields, they merge simplicity and scalability. The XS Technologies® platform can help accommodate industry trends, eliminate production bottlenecks, align downstream processing and drive breakthroughs into the clinic.

XS® Pichia Expression System

Lonza’s XS® Pichia platform is best in class for complex, non-glycosylated protein production using a Pichia pastoris expression system. Its features include:

• Bacteria-like short fermentation time
• Simple and fast strain development
• Methanol-free fermentation
• No viral clearance required
• Highly pure product
• Protein secretion
• No endotoxin

XS® E.coli Expression System

E.coli is one of the most trusted and established organisms for the expression of simple to moderately complex biologics. The features of our E.coli expression system include:

• Reduced requirement for antibiotics
• Robust and scalable upstream processes
• Tunable expression to simplify downstream processing
• High titers
• Baseline expression does not need to repressed

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Pichia bridges the gap between bacterial and mammalian expression systems

The Pichia pastoris expression system is a versatile expression system that combines the advantages of other commonly used platforms. Like E.coli, Pichia strains are simple, fast to develop and achieve high yields in reasonable fermentation times with no need for viral clearance. Pichia is free of endotoxin and secretes a pure, high-quality product, eliminating the need to lyse cells.

Our glucose-inducible and methanol-free XS® Pichia system is designed to reduce host cell impurities, while folding and assembling more complex proteins to secrete them into the supernatant. Features of this system facilitate ready scalability and simpler downstream processing.

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XS® E.coli enables optimized expression of simple to moderately complex proteins using an E.coli Expression System. Its robust processes help improve productivity and overall speed to market for your biologic.

The XS® E.coli Expression System includes multiple hosts, several signal sequences and three novel induction systems.

Key benefits of these induction systems include:

• Reduced requirement for antibiotics
• Robust and scalable upstream processes
• Tunable expression to simplify downstream processing
• High titers
• Baseline expression does not need to be repressed

Combine three alternative promoter systems with other elements to optimize the expression of your biologics and increase your success rates.

One toolbox of expression system components enables you to screen and make impactful choices.

Diversity of biotherapeutics requires a toolbox approach for expression options. The XS Technologies® platform, (including the XS® Pichia and E.coli Expression Systems) is an extensive expression toolbox. Under license, you can use this platform in your lab and access Lonza’s support where you need it the most. It is the same highly productive and scalable platform we use for our in-house services - so you can be confident in its ability.

Host-screening and support from our technical team will help you choose which system would work best for your molecule.

Learn more about Microbial Strain Development or contact our experts