Optimizing expression vectors for biotherapeutic protein production: design principles, titer-influencing factors and promoters

The efficient production of biotherapeutic proteins hinges on the design and optimization of expression vectors. This article explores the key principles underlying vector design for maximizing protein yield, stability, and functionality in recombinant systems. It provides a comprehensive analysis of vector components, including promoters, enhancers, untranslated regions, and selection markers, with a special emphasis on novel promoter development at Lonza and how this can help to solve industry challenges associated with expressing a range of biotherapeutic proteins.