Generating a bispecific antibody, which is correctly and stably paired, is a major production concern. Many solutions require significant changes to native antibody structure, which increases antibody complexity and forces adaptation of downstream processes. While a various platforms have been developed to mitigate Heavy-Light chain (HC-LC) mispairing, there are many other rate limiting steps for efficiently expressing these molecules in a CHO system. bYlok® technology is a design engineering approach that stabilises the interaction between the HC and LC, essentially removing the mispairing problem whilst retaining a more natural antibody structure. The presentation will also include how the Lonza GS System® is supports development of bispecific molecules by streamlining vector construction process and improved titers using GS piggyBac® transposon system.
Author: Lisa Prendergast, Ph.D Associate Director, Expression System Sciences