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Reporter gene technologies are excellent tools to study gene expression, regulation as well as complex cellular processes such as gene networks. Reporter systems based on luciferase represent a highly sensitive, simple and flexible method which is especially suited for high throughput screenings.

The Nucleofector™ Technology provides easy and reproducibly efficient transfection of almost any cell type in single reactions or up to 384-wells at a time. In combination with Promega's luciferase reporter technology it leads to rapid and high expression even in scientifically most relevant primary cells. For transfection optimization luciferase reporter technology is a well suited alternative to GFP, especially for high throughput applications.

Benefit from

  • Luciferase expression within just a few hours
  • Working with primary cells or cell lines
  • Easy optimization of transfection conditions
  • Quantitative analysis using standard plate readers

Nucleofection for Luciferase assays
Nucleofection™ enables luciferase assays in primary cells. Human Mesenchymal Stem Cells (HMSC) and Human Umbilical Vein Endothelial Cells (HUVEC) were transfected by Nucleofection™ with a firefly luciferase expression plasmid. Luciferase expression was analyzed 6, 24 and 48 hours post Nucleofection™ by Dual-Glo Assay (Promega). The 24 h value was set to 1, values are presented as 10-1 RLUs.