Photo of A. Henn sitting at a workstation from Biospherix

Alicia Henn, CSO, BioSpherix Ltd.

Full-time Control of Conditions during Cell Handling Enhances the Expansion of Human MSC for Stem Cell Therapies

Research Interest

BioSpherix’s scientific laboratory explores Cytocentric™ environments, in vitro spaces designed so that cells are protected from room air at all times, even during cell handling. A controlled environment is essential for good science, yet the conventional open room laboratory provides highly unstable and non-physiologic conditions for cells. There is a high risk of microbial contamination and there are irregular exposures of cells to room air which is too cold, too low in carbon dioxide, and far too high in oxygen for cells to function as they do in vivo. As the high failure rate for drug candidates shows, it is very difficult to translate cellular responses assessed in room air environments to the in vivo environment. We strive to make the in vitro environment a more constant, less stressful, and more physiologically relevant place for cells.

The Experiment

In this study, we used the CytoSMART™ System to produce time-lapse photos and cell density graphs of cells grown under full-time optimal conditions in the Xvivo System X3 or under more traditional, non-optimized laboratory conditions. We used Human Bone Marrow Mesenchymal Stromal Cells (Lonza), a cell type that is highly valued for its wide-ranging clinical applications.


Video of hMSC recorded with the CytoSMART™ Lux 10X Device (Snapshot interval: 15 minutes)   Analysis of cell confluency data recorded with the CytoSMART™ System: Under full-time optimal conditions for both incubation and handling (blue graph), human MSC grew to higher yields as compared to cells exposed to room air conditions for routine passage (grey graph).


The CytoSMART™ System was the perfect partner for our cytocentric studies, providing real-time readouts on cell growth throughout the experiments without disturbing the cells, in every environment. We could check the cells from anywhere, even on the weekends, to see how cell growth was progressing under the different conditions. We would encourage anyone concerned about the effect of interrupted conditions on their cells to use the CytoSMART™ System to get data on cell growth in place.  

Find out more about Alicia Henn’s work and download the White Paper on the impact of physioxic cell culture conditions.

 Learn more and download the White Paper