In a previous paper, a method was suggested for isolation of nutritionally deficient mutants in maammalian cells (Fig. 1). A larger cell population is exposed to 5-brumodeoxyuridine (BUdR) in a medium lacking certain nitrilites. Those cells competent to grow in a given medium incorporate BUdR into their DNA and are subsequently killed by an exposure to near-visible light. The deficient mutants do not incorporate the brominated analogue and are unaffected by the illumination. These are then grown up into colonies by replacement of the nutitionally deficient medium with one enriched with various metabolites. The resulting colonies can be isolated and their nutritional requirements determined. The present report describes application of this method to the induction and isolation of mutants of Chinese hamster cells.
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