Mycoplasma Contamination:
Causes, Problems, Solutions

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The MycoAlert™ PLUS Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells.

 Convenient &  fast
  • No DNA extraction necessary
  • Simply add  two reagents and perform two luminescence readings
  • Easy interpretation of results


  • Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)
  • No interference with bacteria, fungi or yeast




NEW! Higher light output with MycoAlert™ PLUS Assay

  • For use with less sensitive plate luminometers or multifunctional readers
  • Allows testing of unused media, media supplements or water



Assay principle:

Viable mycoplasma in a test sample (cell supernatant, fresh media or supplements) are lysed and the enzymes react with the MycoAlert™ PLUS substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert™™ PLUS Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert™ PLUS Substrate (read B), a ration can be obtained which is indicative of the presence or absence of mycoplasma.

 MycoAlert Protocol Scheme