What is the RAFT™ 3D Cell Culture System?

RAFT™ 3D Cell Culture Kit

  • RAFT™ System – A Complete Solution

    The novel RAFT™ 3D Cell Culture System is designed to closely mimic the native environment of cells. The RAFT™ 3D Cell Culture Kit offers a complete solution with the necessary reagents and consumables to develop RAFT™ 3D Cultures.

     

    The RAFT™ System uses rat tail collagen type 1 along with patented absorber technology to create a realistic cellular environment to study cells' complex behavior. RAFT™ Kit is a simple-to-use 3D cell culture system that enables the production of robust, reproducible 3D cultures in less than an hour.

     

     How it works

    3D culture with RAFT System - Process visualization

     

    Watch the video

         

    What does the RAFT™ System Consist of?

    • RAFT™ Reagent Kit – consists of rat tail collagen 1 as the extracellular matrix scaffold and a specially-designed neutralizing solution that enables neutralization to be carried out in a single step instead of an error-prone and tedious titration method. The kit also contains 10X MEM to support cells through the RAFT™ Process.

    • Novel RAFT™ Absorbers – The biocompatible absorbers are designed to work with standard 96- or 24-well cell culture plates as well as 24-well cell culture inserts. Once the cells are mixed with RAFT™ Reagents, the absorbers are utilized to compress and absorb excess liquid from the collagen mixture. This results in the creation of well-controlled, high-density collagen scaffolds embedded with cell type(s) of choice. The RAFT™ System is one of the few commercially available kits that allows users to create these high-density collagen scaffolds that are representative of a natural, more in vivo like environment for cells to grow and interact more efficiently.
     

    Acellular RAFT™ 3D Cultures

    Floating acellular RAFT™ 3D Cultures

     

    Cells are embedded inside collagen scaffold for tissue modeling and long-term culture




    Webinar – By Biotechniques

    Learn how three scientists are using the RAFT™ 3D System to create and analyse complex 3D tissue models.                                           

    Watch on Demand

       

    RAFT™ 24-well Insert Kit for Cell Culture Inserts

    (catalog number 016-1R25 and 016-1R33)

    RAFT™ Insert Absorbers are designed to be used with 24-well permeable membrane cell culture inserts.

      RAFT™ Insert Absorbers for 24-well plate

    This additional kit is offered to enhance the versatility of the system further by allowing researchers to generate co-culture models, and barrier models including air-lift models. 

    Please note no cell culture inserts are supplied within the RAFT™ 24-well Insert Kit due to variety in pore size. Please contact Scientific Support for a detailed list of RAFT™ Kit compatible inserts or visit our FAQ section.


     

     

    Ways to set up RAFT™ System

    View or download PDF

     

    Is the RAFT™ System applicable to your project?

     

    Contact us to find out

           

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