Benefits of the
for siRNA applications:
- Proven transfection technology with more than 300 siRNA publications
- siRNA delivery with up to 99% efficiency in primary cells and difficult-to-transfect cell lines such as suspension cells
- Low cytotoxicity
- Co-transfection with plasmid DNA for transfection control or rescue experiments
- siRNA library screening in difficult-to-transfect cell types using the 96-well Shuttle™ or HT Nucleofector™ Systems
Nucleofection™ outperforms lipofection for effective GAPDH mRNA knockdown in difficult-to-transfect cell types. Cells were transfected with 5 pmol SMARTpool® reagent (Dharmacon) targeting GAPDH using the 96-well Shuttle™ System (according to the respective optimized protocol) or Reagent L (after titration of optimal reagent amount). GAPDH mRNA levels were analyzed 24 hours post-transfection by the QuantiGene branched-DNA assay (Panomics) and normalized to siCONTROL® Non-Targeting siRNA #1 (Dharmacon).