MicroRNAs (miRNAs) are endogenous, small, non-coding RNA molecules which were shown to be key regulators of gene expression at the level of translation. They are differentially expressed in tissues, critical in the development of organisms, involved in viral infection, and associated with oncogenesis.
As with any other gene classes, their functionality is analyzed by overexpression using transfection of synthetic miRNAs or miRNA-expressing plasmids or by down-regulation using transfection of miRNA inhibitors.
Benefits of the Nucleofector™ Technology
for miRNA applications:
- Allows studying miRNAs in difficult-to-transfect cells including suspension cell lines and primary cells
- More than 90% transfection efficiency for both, RNA oligonucleotides and DNA vectors
HT Nucleofector™ System or 96-well Shuttle™ System enabling library screening
Expression of miR132 induces neurite sprouting by targeting a protein that represses neurite outgrowth (p250GAP). Rat neonatal cortical neurons were transfected with a GFP reporter (green) and co-transfected with vector control, or expression constructs for premiR1-1 or premiR132 using Rat Neuron Nucleofector™ Kit and Nucleofector™ II/2b Device. Cells were immunostained for the neuronal marker MAP2 (red). Only cells transfected with premiR132 show neurite sprouting.