Labelled siRNA transfected into HL-60

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Gene silencing by RNA interference (RNAi) emerged as a powerful technology for assessing gene function in mammalian cells, and has become a valuable tool in functional genomics, target discovery and validation. The two most critical factors determining the effectiveness of RNAi experiments are the ability of the siRNA sequence to specifically silence the target mRNA, and the efficiency with which siRNA (or an shRNA-expressing vector) can be transfected into the cells of interest.



Benefits of the Nucleofector™ Technology for RNAi applications:
 

  • Proven delivery method with over 400 RNAi related publications
  • Enables RNAi experiments in difficult-to-transfect cell types, e.g. Jurkat or primary neurons
  • High transfection efficiencies for different substrates, e.g. RNA oligonucleotides (siRNA, synthetic miRNA, miRNA inhibitors) and shRNA- or miRNA-expressing vectors
  • Same protocol for different substrates allows easy switch between substrates and co-transfection of different substrates
  • 96-well Shuttle™ Add-On and the HT Nucleofector™ System as high-throughput platforms for RNAi screenings
  • No reagent-induced cytotoxicity
  • No lipid-induced induction of interferon responses1 or off-target effects2

 

1) Marques JT & Williams RG (2005) Nat Biotechnol 23(11):1399-405
2) Fedorov Y et al. (2005) Nat Methods 2(4):241