Reporter gene technologies are excellent tools to study gene expression, regulation as well as complex cellular processes such as gene networks. Reporter systems based on luciferase represent a highly sensitive, simple and flexible method which is especially suited for high throughput screenings.
The Nucleofector™ Technology provides easy and reproducibly efficient transfection of almost any cell type in single reactions or up to 384-wells at a time. In combination with Promega's luciferase reporter technology it leads to rapid and high expression even in scientifically most relevant primary cells. For transfection optimization luciferase reporter technology is a well suited alternative to GFP, especially for high throughput applications.
- Luciferase expression within just a few hours
- Working with primary cells or cell lines
- Easy optimization of transfection conditions
- Quantitative analysis using standard plate readers
Nucleofection™ enables luciferase assays in primary cells. Human Mesenchymal Stem Cells (HMSC) and Human Umbilical Vein Endothelial Cells (HUVEC) were transfected by Nucleofection™ with a firefly luciferase expression plasmid. Luciferase expression was analyzed 6, 24 and 48 hours post Nucleofection™ by Dual-Glo Assay (Promega). The 24 h value was set to 1, values are presented as 10-1 RLUs.