GENERAL Amount Unit Default
number of transfections # 96
cells / transfection #  
void volume comp. 20µl + X % % 20
 
SUBSTRATES
pmaxGFP™ / reaction µg  
pmaxGFP™ concentration (stock) µg/µl  
DNA / transfection µg  
DNA concentration (stock) µg/µl  
siRNA / transfection in µg µg  
siRNA concentration (stock) µg/µl  
siRNA / transfection in µM µM  
siRNA concentration (stock) µM  
 
SUBSTRATE VOLUMES
pmaxGFP™ (per well) µl  
pmaxGFP™ (total) µl  
DNA (per well) µl  
DNA (total) µl  
siRNA (µg-based, per well) µl  
siRNA (µg-based, total) µl  
siRNA (µM-based, per well) µl  
siRNA (µM-based, total) µl  
 
SOLUTIONS
Nucleofector™ Solution µl  
Supplement µl  
total volume µl  
 
CELLS
cell number in total volume #  
 
RINSING MEDIUM POST TRANSFECTION
medium per well post transfection µl 80
min. vol. of prewarmed medium ml  
 
CELL CULTURE POST TRANSFECTION
transfer volume to cell culture µl 25/50
total cell culture volume µl  
preplated medium volume per well µl