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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of mouse ES cells using the different Nucleofection™ Platforms. Optimal kits for transfection of mouse ES cells in the 4D-Nucleofector™ and 96-well Shuttle™ are the P3 Primary Cell Kits used in combination with a cell-type specific protocols. Mouse ES cell specific kits are available for the Nucleofector™ II/2b Device.
Example for the Nucleofection™ of mouse ES cells with lacZ reporter
Mouse ES cells were transfected by Nucleofection™ with a lacZ reporter and stained 48 hours after transfection for transient lacZ expression. The picture shows a mostly homogenous lacZ staining. This result was repeatedly achieved in up to 80% of the transfected cells.
(Data courtesy of S. Boljahn et al., amaxa application note, Sep. 2004).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuvette™ Strip.