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For human stem cells lacking a cell-type specific Amaxa™ Optimized Protocol, we offer a selection of kits that can be used to easily define optimal Nucleofection™ Conditions. The P3 and P4 Primary Cell Kits together with the cellgroup specific Basic Protocols are suited for optimizations of human stem cells on the 4D-Nucleofector™ System and the 96-well Shuttle™. A cell-group specific Basic Kit is suited for optimization of human stem cells using the Nucleofector™ II/2b Device.
Average transfection efficiency of human stem cell lines
Different human stem cell lines were transfected by Nucleofection™ using pmaxGFP™ Vector according to the guidelines given in the human Stem Cell Nucleofector™ Starter Kit. (Data for Nucleofection™ of human stem cells are compiled from experiments performed by leading stem cell research customers.)
For the 4D-Nucleofector™ Kits "L" and "S" indicate the
Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuvette™ Strip.