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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of H9 human stem cells using the different Nucleofection™ Platforms. Optimal kits for transfection of human H9 stem cells in the 4D-Nucleofector™ and 96-well Shuttle™ System are the P3 Primary Cell Kits used in combination with cell-type specific protocols. A stem cell specific Basic Kit is suited for optimization of human stem cells using the Nucleofector™ II/2b Device.
H9 cells preserve pluripotency post Nucleofection™
H9 cells transfected by Nucleofection™ with pmaxGFP™ Vector maintain their undifferentiated state. Analysis after 24 hours shows expression of maxGFP™ Protein (green) as well as of the pluripotency markers SSEA4 (red) and Oct4 (purple). The blue signals refer to nuclear staining by DAPI.
(Data kindly provided by Jennifer Moore, Rutgers University, Piscataway, USA).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuvette™ Strip.