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For mammalian smooth muscle cells lacking a cell-type specific Amaxa™ Optimized Protocol, we offer a selection of kits that can be used to easily define optimal Nucleofection™ Conditions. The P1 Primary Cell Kits together with the cell-group specific Basic Protocols are suited for optimizations of mammalian smooth muscle cells on the 4D-Nucleofector™ System and the 96-well Shuttle™ System. A cell-group specific Basic Kit is suited for optimization of mammalian smooth muscle cells using the Nucleofector™ II/2b Device.
Nucleofection™ of primary smooth muscle cells
Primary pulmonary artery smooth muscle cells were transfected with pmaxGFP™ Vector. 24 hours post Nucleofection™, cells were analyzed by light (left) and fluorescence (right) microscopy.
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuvette™ Strip.