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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of human AoSMCs using the different Nucleofection™ Platforms. Optimal kits for transfection of human AoSMCs in the 4D-Nucleofector™ and 96-well Shuttle™ System are the P1 Primary Cell Kits used in combination with respective basic protocols for mammalian smooth muscle cells. Human AoSMC specific kits are available for the Nucleofector™ II/2b Device.
Time course of transient expression of transfected human AoSMC
Human AoSMC were transfected by Nucleofection™ using a plasmid encoding the mouse MHC class I heavy chain molecule H-2Kk. 1, 2, 7, 14, 21, and 28 days post Nucleofection™, the cells were analyzed for their H-2Kk expression by flow cytometry. Dead cells were excluded from the analysis by propidium iodide staining and gating.
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuvette™ Strip.