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A selection of kits for mammalian glial cells helps you to easily define optimal Nucleofection™ Conditions. The P3 Primary Cell Kits together with the cell-group specific Basic Protocols are suited for optimizations of mammalian glial cells on the 4D-Nucleofector™ and the 96-well Shuttle™ System. A cell-group specific Basic Kit is suited for optimization of mammalian glial cells using the Nucleofector™ II/ Device. Using the new 4D-Nucleofector™ Y Unit, Nucleofection™ of glial cells can be performed in an adherent state using AD1 4D-Nucleofector™ Y Kit.
Expression of GFAP and eGFP in rat astrocytes transfected by Nucleofection™
Primary rat astrocytes were isolated from rat embryos (E17) and cultured for 10 days until cells reached confluency. These cells were transfected with a plasmid encoding the enhanced green fluorescent protein, eGFP. 24 hours post Nucleofection™, cells were analyzed by fluorescence microscopy for expression of eGFP (A) and GFAP (B), an astrocyte-specific marker protein. Nuclei were stained with DAPI (C). D shows an overlay of the images. (Photographs courtesy of Dr. Hyun-Ju Kim and Dr. Tim Vartanian, Beth Israel Deaconess Medical Center, Dept. of Neurology, Boston, Massachusetts, USA.)
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuvette™ Strip.