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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of mouse or rat hepatocytes using the different Nucleofection™ Platforms. For the transfection of mouse or rat hepatocytes in the 4D-Nucleofector™ or 96-well Shuttle™ System we recommend using the Primary Cell Optimization Kits and the respective optimization protocols. Optimal Nucleofection™ Conditions are transferable between these systems. A mouse and rat hepatocyte specific kit is available for the Nucleofector™ II/ Device.
Nucleofection™ of primary mouse hepatocytes
Mouse hepatocytes were transfected by Nucleofection™ with pmaxGFP™ Vector. 24 hours post Nucleofection™ cells were analyzed by light (upper picture) and fluorescence microscopy (lower picture).