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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of human hepatocytes using the different Nucleofection™ Platforms. Optimal kits for transfection of human hepatocytes in the 4D-Nucleofector™ and 96-well Shuttle™ System are the P3 Primary Cell Kits used in combination with cell-type specific protocols.
Example showing typical Nucleofection™ results of human hepatocytes
Cryopreserved human hepatocytes were transfected by Nucleofection™ with pmaxGFP™ Vector. 120 hours post Nucleofection™ cells were analyzed by light (Left) and fluorescence microscopy (Right).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.