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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of MEFs using the different Nucleofection™ Platforms. For the transfection of primary MEFs in the 4D-Nucleofector™ and 96-well Shuttle™ System we recommend using the P4 Primary Cell Kits used in combination with a cell-type specific protocol. For the Nucleofector™ II/2b Device specific kits for transfection of immortalized MEFs are available. Furthermore we have a Nucleofector™
II/2b Device Protocol available for transfection of primary, non-immortalized MEFs.
Nucleofection™ of primary MEF
Primary mouse embryonic fibroblasts were transfected using pmaxGFP™ Vector. Cells were analyzed 24 hours post Nucleofection™ using fluorescence.
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.