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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of NHDF cells using the different Nucleofection™ Platforms.Optimal kits for transfection of NHDF cells in the 4D-Nucleofector™and 96-well Shuttle™ System are the P2 Primary Cell Kits used in combination with cell-type specific protocols. NHDF cell specific kits are available for the Nucleofector™ II/2b Device.
Nucleofection™ of adult human dermal fibroblasts with eGFP cDNA
Adult NHDF were transfected by Nucleofection™ using a plasmid encoding eGFP. 24 hours post Nucleofection™, cells were analyzed by light (left) and fluorescence microscopy (right).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.