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For mammalian endothelial cells lacking a cell-type specific Amaxa™ Optimized Protocol, we offer a selection of kits that can be used to easily define optimal Nucleofection™ Conditions. The P5 Primary Cell Kits together with the cell group-specific Basic Protocols are suited for optimizations of mammalian endothelial cells on the 4D-Nucleofector™ and 96-well Shuttle™ System. A cell-group specific Basic Kit is suited for optimization of mammalian endothelial cells using the Nucleofector™ II/2b Device.
Example for Nucleofection™ of primary porcine endothelial cells
Primary porcine trabecular meshwork cells (derived from eye) were transfected by Nucleofection™ with a plasmid encoding the green fluorescent maxGFP™ Reporter Protein. 24 hours post Nucleofection™ the cells were analyzed by light (left) and fluorescence microscopy (right).
(Data courtesy of Dr. Ted Acott, Oregon Health & Science University, USA.)
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.