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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of HUVECs using the different Nucleofection™ Platforms. Optimal kits for transfection of HUVECs in the 4D-Nucleofector™ and 96-well Shuttle™ System are the P5 Primary Cell Kits used in combination with cell-type specific protocols. HUVEC specific kits are available for the Nucleofector™ II/2b Device.
Nucleofection™ of HUVEC
HUVEC were transfected by Nucleofection™ with the pmaxGFP™ Vector. 24 hours post Nucleofection™ cells were analyzed by light microscopy (left) or fluorescence microscopy (right).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.