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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of HMVEC-L using the different Nucleofection™ Platforms. Optimal kits for transfection of HMVEC-L in the 4D-Nucleofector™ and 96-well Shuttle™ System are the P5 Primary Cell Kits used in combination with respective basic protocols for mammalian endothelial cells. HMVEC-L specific kits are available for the Nucleofector™ II/2b Device.
Example for the Nucleofection™ of HMVEC-L
HMVEC-L were transfected by Nucleofection™ using a plasmid encoding the enhanced green fluorescent protein eGFP. 24h post Nucleofection™ cells were analyzed by light (A) and fluorescence microscopy (B).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuvette™ Strip.