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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of HCAECs using the different Nucleofection™ Platforms. Optimal kits for transfection of HCAECs in the 4D-Nucleofector™ and 96-well Shuttle™ System are the P5 Primary Cell Kits used in combination with respective Basic Protocols for mammalian endothelial cells. For the Nucleofector™ II/2b Device HCAEC specific kits are available.
Nucleofection™ of HCAEC
HCAEC were transfected by Nucleofection™ with a plasmid encoding the fluorescent protein. 24 hours post Nucleofection™ cells were analysed by light (left) and fluorescence microscopy (right).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.