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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of human melanocytes using the different Nucleofection™ Platforms. For the transfection of human melanocytes in the 4D-Nucleofector™ System and the 96-well Shuttle™ System we recommend using the Primary Cell Optimization Kits and the respective optimization protocols. For the Nucleofector™ II/2b Device human melanocyte specific kits are available.
Nucleofection™ of NHEM-Neo with eGFP cDNA
NHEM-Neo were transfected by Nucleofection™ using a plasmid encoding enhanced green fluorescent protein eGFP. 24h post Nucleofection™ cells were analyzed by light (A) and fluorescence microscopy (B).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.