Download our Catalog and Resource Notes
Customer Service or Scientific Support
Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of human keratinocytes using the different Nucleofection™ Platforms. Optimal kits for transfection of human Keratinocytes in the 4D-Nucleofector™ and 96-well Shuttle™ System are the P3 Primary Cell Kits used in combination with cell-type specific protocols. Human keratinocyte specific kits are available for the Nucleofector™ II/2b Device.
Nucleofection™ of primary human neonatal keratinocytes
Human neonatal keratinocytes isolated from foreskin were transfected by Nucleofection™ with a plasmid encoding the enhanced green fluorescent protein eGFP. Cells were analyzed 24 hours post Nucleofection™ by light (left) and fluorescence microcopy (right).