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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of human chondrocytes using the different Nucleofection™ Platforms. Optimal kits for transfection of human chondrocytes in the 4D-Nucleofector™ and 96-well Shuttle™ are the P3 Primary Cell Kits used in combination with a cell-type specific protocol. For the Nucleofector™ II/2b Device human chondrocyte cell specific kits are available.
Nucleofection™ of primary human chondrocytes
Human chondrocytes were transfected by Nucleofection™ with a plasmid encoding the enhanced green fluorescent protein eGFP. Cell membranes were fluorescently stained in red with the substance R18 (Octadecylrhodamine-B-chloride, Molecular Probes). The image shows an overlay of eGFP and R18 fluorescence.