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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of stimulated mouse B cells using the different Nucleofection™ Platforms. Optimal kits for transfection of mouse B cells in the 4D-Nucleofector™, 96-well Shuttle™ and HT Nucleofector™ System are the P4 Primary Cell Kits used in combination with cell-type specific protocols. Mouse B cell specific kits are available for the Nucleofector™ II/2b Device.
Example showing typical transfection results of stimulated Mouse B cells
Mouse B cells were stimulated with LPS for 24 hours to induce blast formation. Stimulated cells were transfected by Nucleofection™ with pmaxGFP™ Vector. 24 hours post Nucleofection™ cells were analyzed by light (A) or fluorescence microscopy (B).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.