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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of mouse T cells using the different Nucleofection™ Platforms. Optimal kits for transfection of mouse T cells in the 4D-Nucleofector™ and 96-well Shuttle™ System are the P3 Primary Cell Kits used in combination with cell-type specific protocols. Mouse T cell specific kits are available for the Nucleofector™ II/2b Device.
Average transfection efficiencies after Nucleofection™ of primary mouse T cells from C57BL/6 and BALB/c mice (CD4)
For the 4D-Nucleofector™ its "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.