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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of mouse macrophages using the different Nucleofection™ Platforms. For the transfection of mouse macrophages in the 4D-Nucleofector™ and 96-well Shuttle™ System we recommend using the Primary Cell Optimization Kits and the respective optimization protocols. Optimal Nucleofection™ Conditions are transferable between these three systems. Mouse macrophage specific kits are available for the Nucleofector™ II/2b Device.
Example for Nucleofection™ of mouse macrophages with pmaxGFP™ Vector
Primary mouse macrophages (isolated from C57BL/6 mice) were transfected by Nucleofection™ with the pmaxGFP™ Vector and analyzed by light (left) and fluorescence microscopy (right) at 40x magnification. Transfected macrophages reveal cytoplasmic extrusions important for phagocytic function of macrophages.