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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of mouse dendritic cells using the different Nucleofection™ Platforms. Optimal kits for transfection of mouse dendritic cells in the 4D-Nucleofector™, 96-well Shuttle™ and HT Nucleofector™ System are the P3 Primary Cell Kits (for mature DCs) and P4 Primary Cell Kits (for immature DCs) used in combination with cell-type specific protocols. Mouse dendritic cell specific kits are available for the Nucleofector™ II/2b Device.
Transfection Efficiency, Viability and Functionality of mouse DC post Nucleofection™
Left graph: Mouse DC (Balb/C) were transfected using pmaxGFP™ Vector. Cells were analyzed 24 hours post Nucleofection™ by flow cytometry for maxGFP™ reporter protein expression and viability. Cell viability is given in percent compared to non-trasnfected control.
The right graph displays functionality of immature moue DCs (isolated from mouse strain Balb/C) post Nucleofection™ (Sample). Two hours post Nucleofection™, cells were stimulated by LPS. 22 hours later, functionality was analyzed by IL-6 specific ELISA and is given in percent compared to non-transfected control.
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.