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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of human NK cells using the different Nucleofection™ Platforms. Optimal kits for transfection of human monocytes in the 4D-Nucleofector™ and 96-well Shuttle™ System are the P3 Primary Cell Kits used in combination with cell-type specific protocols. Human monocyte specific kits are available for the Nucleofector™ II/2b Device.
Nucleofection™ of primary human NK cells
Primary human natural killer cells were transfected by Nucleofection™ with a plasmid encoding the enhanced green fluorescent protein eGFP. Cells were analyzed by flow cytometry 24 hours post Nucleofection™. eGFP expression in natural killer cells is shown after Nucleofection™ without (A) and with plasmid DNA (B). (Courtesy of J. Sundbäck and K. Kärre, Karolinska Institutet, Microbiology and Tumor Biology Center, Stockholm, Sweden).