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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of human macrophages using the different Nucleofection™ Platforms. Optimal kits for transfection of human macrophages in the 4D-Nucleofector™, 96-well Shuttle™ and HT Nucleofector™ System are the P3 Primary Cell Kits used in combination with cell-type specific protocols. Human macrophage specific kits are available for the Nucleofector™ II/2b Device.
Nucleofection™ of primary human macrophages
Primary human macrophages were transfected by Nucleofection™ with the pmaxGFP™ Vector and analyzed by light (right) and fluorescent microscopy (left) at 40x magnification. Transfected macrophages reveal cytoplasmic extrusions important for phagocytic function of macrophages.
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.