Download our Catalog and Resource Notes
Customer Service or Scientific Support
Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of human dendritic cells using the different Nucleofection™ Platforms. For the transfection of human dendritic cells in the 4D-Nucleofector™ and 96-well Shuttle™ System we recommend using the Primary Cell Optimization Kits and the respective optimization protocols. Optimal Nucleofection™ Conditions are transferable between these three systems. Human dendritic cell specific kits are available for the Nucleofector™ II/2b Device.
Nucleofection™ of human dendritic cells
Human dendritic cells were differentiated from monocytes using 100ng/ml IL-4 and 50 ng/ml GM-CSF. On day 6 cells were transfected by Nucleofection™ with plasmid DNA encoding the green fluorescent protein (eGFP). 24 hours post Nucleofection™ cells were analyzed by flow cytometry (FACS). FACS data show mock transfected cells (plasmid without insert) (A) and eGFP transfected cells (B). (Courtesy of Dr. Esen and Prof. Radbruch Deutsches Rheuma-Forschungszentrum Berlin (DRFZ), Germany).