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Parasitic protozoa infect vertebrates and invertebrates and some are even parasitic in plants. In humans they can cause severe diseases such as Malaria (Plasmodium), Sleeping Sickness (Trypanosoma) or Leishmaniasis (Leishmania). Nucleofection™ has proven to provide strongly increased transfection efficiencies (e.g. in Plasmodium berghei and Trypanosoma brucei) compared to standard methods such as electroporation or particle bombardment. Due to significant genotypic and phenotypic diversity between species and life cycles Lonza has developed two Basic Parasite Nucleofector™ Kits (1 and 2) and an easy-to-use Basic Parasite Nucleofector™ Starter Kit.
For the transfection of parasites using 4D-Nucleofector™ or 96-well Shuttle™ we recommend using our Primary Cell Optimization Kits.