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Each kit contains
The initial optimization experiment is comprised of 80 reactions. Five different Primary Cell 4D-Nucleofector™ Solutions P1-P5 are each tested in combination with 15 different Nucleofector™ Programs plus one control. The Nucleofection™ Condition with the highest efficiency and lowest mortality is selected for all subsequent experiments and can be also used in the 100 μl single Nucleocuvette™.