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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of THP-1 cells using the different Nucleofection™ Platforms.
Nucleofection™ of THP-1 cells
THP-1 cells were transfected by Nucleofection™ using a plasmid encoding the enhanced green fluorescent protein eGFP. 5 hours post Nucleofection™, the cells were analyzed by light (top) and fluorescent microscopy (bottom).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuvette™ Strip.