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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of Sf9 cells using the different Nucleofection™ Platforms.
Example for Nucleofection™ of Sf9 cells
Sf9 cells were transfected by Nucleofection™ using an insect expression vector encoding maxGFP™ Reporter protein. 48 hours post Nucleofection™ , the cells were analyzed by light (A) and fluorescence microscopy (B).
The pmaxGFP™ Vector provided in our Nucleofector™ Kits is not expressed in insect cells!
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.