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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of NIH/3T3 cells using the different Nucleofection™ Platforms.
Different NIH/3T3 subclones derived from different sources may have divergent features. Due to this variety between different NIH/3T3 clones optimal Nucleofection™ Conditions may vary for each clone. This is the reason why we developed different protocols for the NIH/3T3 clone CRL-1658 from American Type Culture Collection and the NIH/3T3 clone ACC49 from DSMZ. For Nucleofection™ of other NIH/3T3 clones, the optimal Nucleofection™ Conditions can easily be determined using the Cell Line Optimization Kits or alternatively please contact our Scientific Support Team for further information.
Example for Nucleofection™ of NIH/3T3 cells
NIH/3T3 cells were transfected by Nucleofection™ using a plasmid encoding the enhanced green fluorescent protein eGFP. 5 hours post Nucleofection™, the cells were analyzed by light (left) and fluorescence microscopy (right).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuvette™ Strip.