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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of K-562 cells using the different Nucleofection™ Platforms.
Different K-562 subclones derived from different sources may have divergent features and display an extremely high genetic instability. Due to this variety between different K-562 clones optimal Nucleofection™ Conditions may vary for each clone. This is the reason why we developed different protocols for the K-562 clone CCL-243 from American Type Culture Collection and the K-562 clone ACC10 from DSMZ. For Nucleofection™ of other K-562 clones, the optimal Nucleofection™ Conditions can easily be determined using the Cell Line Optimization Kits or alternatively please contact our Scientific Support Team for further information.
Nucleofection™ of K-562 cells
K-562 cells were transfected by Nucleofection™ using a plasmid encoding the enhanced green fluorescent protein eGFP. 5 hours post Nucleofection™, the cells were analyzed by light (top) and fluorescence microscopy (bottom).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuvette™ Strip.