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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of Jurkat cells using the different Nucleofection™ Platforms.
Different Jurkat subclones derived from different sources may have divergent features and display an extremely high genetic instability. Due to this variety between different Jurkat clones optimal Nucleofection™ Conditions may vary for each clone. We strongly recommend the use of the Jurkat clones E6-1 (American Type Culture Collection) or ACC 282 (DSMZ). For Nucleofection™ of other Jurkat clones, the optimal Nucleofection™ Conditions can easily be determined using the Cell Line Optimization Kits or alternatively please contact our Scientific Support Team for further information.
Gene transfer efficiencies and viability of Jurkat cells
E6-1 (American Type Culture Collection) or ACC 282 (DSMZ) Jurkat cells were transfected by Nucleofection™ using pmaxGFP™ Vector. 24 hours post Nucleofection™ the cells were analyzed by FACS analysis for maxGFP™ Reporter protein expression. Cell viability was determined as PI negative cells.
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuvette™ Strip.