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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of Hep G2 cells using the different Nucleofection™ Platforms.
Example for Nucleofection™ of Hep G2 cells with H-2Kk cDNA
Hep G2 cells were transfected by Nucleofection™ using a plasmid encoding the mouse MHC class I heavy chain molecule H-2Kk. 24 hours post Nucleofection™, the cells were stained with a PE-coupled antibody directed against H-2Kk and were analyzed by flow cytometry. HepG2 cells were gated according to forward/side scatter (A). Dead cells were excluded by staining with propidium iodide and gating (B). H-2Kk expression is shown after Nucleofection™ without (C) and with plasmid DNA (D).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuvette™ Strip.