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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of HaCaT cells using the different Nucleofection™ Platforms.
Average transfection efficiency of HaCaT cells
HaCaT cells were transfected by Nucleofection™ using a plasmid encoding the enhanced green fluorescent protein eGFP. 24 hours post Nucleofection™, the cells were analyzed by flow cytometry. (Courtesy of Dr. T. Wachter and PD Dr. M. Leverkus, Dermatology Department, University of Wuerzburg, Germany).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.