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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of Cos7 cells using the different Nucleofection™ Platforms.
Transfection efficiency and viability of COS-7 cells 24 hours post Nucleofection™
COS-7 cells (American Type Culture Collection, CRL-1651 on the left; DSMZ, ACC60 on the right) were transfected by Nucleofection™ using the pmaxGFP™ Vector. Cells were analyzed 24 hours post Nucleofection™ by flow cytometry for GFP expression. Cell viability was analyzed by using CellTiter-Blue Assay (Promega) according to manufacturers instructions.
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.