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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of CHO (suspension) cells using the different Nucleofection™ Platforms.
Average transfection efficiencies and viabilities (% PI-negative) of suspension CHO cells (ECACC)
Cells were transfected by Nucleofection™ using pmaxGFP™ Vector. 24 hours post Nucleofection™ , the cells were analyzed by flow cytometry.
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuevtte™ Strip.