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Various Nucleofector™ Kits and corresponding Amaxa™ Optimized Protocols are available for the transfection of CHO-K1 cells using the different Nucleofection™ Platforms.
Different CHO-K1 subclones derived from different sources may have divergent features and display an extremely high genetic instability. Due to this variety between different CHO-K1 clones optimal Nucleofection™ Conditions may vary for each clone. This is the reason why we developed different protocols for the CHO-K1 clone CCL-61 from American Type Culture Collection and the CHO-K1 clone ACC110 from DSMZ. For Nucleofection™ of other CHO-K1 clones, the optimal Nucleofection™ Conditions can easily be determined using the Cell Line Optimization Kits or alternatively please contact our Scientific Support Team for further information.
Example for Nucleofection™ of CHO-K1 cells
CHO-K1 were transfected by Nucleofection™ using a plasmid encoding the fluorescent protein eGFP. 24 hours post Nucleofection™ , cells were analyzed by light (left) and fluorescence microscopy (right).
For the 4D-Nucleofector™ Kits "L" and "S" indicate the Nucleocuvette™ Vessel format. L = 100 µl single Nucleocuvette™, S = 16-well Nucleocuvette™ Strip.